泱偺攟梴

丂傾僼儕僇僣儊僈僄儖偺弶婜敪惗偵偼丄堦掕偺擹搙偺Na+ K+ Ca2+ Mg2+ 偺僇僠僆儞偑昁梫偱偁傞丅偙傟傜偺僀僆儞偺寚擛傕偟偔偼夁忚偼偲偒偵堎忢側敪惗偺尨場偲側傞丅摉尋媶幒偱偼丄斾妑揑扨弮側慻惉偺墫椶梟塼偺MMR傗僗僞僀儞僶乕僌梟塼傪50亾乣10亾偵敄傔偰巊梡偟偰偄傞丅MMR 偼椉惗椶偺昗弨揑側惗棟揑墫椶梟塼偲傎傏摍丄僗僞僀儞僶乕僌梟塼偼丄50乣60亾偺墫擹搙偱偁傞丅幚嵺偺攟梴偵偼僆乕僩僋儗乕僽柵嬠偟偨傕偺傪梡偄丅応崌偵傛偭偰偼丄峈惗暔幙傪揧壛偡傞丅
丂價僥儕儞枌傪彍偄偨泱偱傕姦揤偺寠偺拞偵偄傟偰偙偺攟梴塼拞偱攟梴偡傟偽丄懡偔偺応崌僞儅僕儍僋僔傑偱惓忢偵敪惗偡傞丅偨偩偟丄泱偺忬懺偵傛偭偰巰偵傗偡偄傕偺偑偁傞丅偺偱拲堄偑昁梫丅傑偨丄泱偵抳柦揑側彎偑偮偔偲丄堎忢棏妱傗丄嵶嬠姶愼偺尨場偲側傞偺偱拲堄偡傞丅娤嶡偺億僀儞僩嶲徠
丂壞応側偳丄嵶嬠姶愼偺嫲傟偺崅偄偲偒偼丄峈惗暔幙傪揧壛偡傞丅摉尋媶幒偱偼丄儁僯僔儕儞偲僗僩儗僾僩儅僀僔儞偺崿塼傪揌壛偟偰偄傞丅偨偩偟丄戝検偵擖傟傞偲丄敪惗偵塭嬁偑弌偰旜夎偺敪惗偑埆偔偰嬋偑偭偰偟傑偭偨傝丄悈朅偑偱偒偨傝偡傞偺偱拲堄丅

丂泱偺庤弍偼gentamycin傪嵟廔擹搙10祃/ml偵側傞傛偆偵壛偊偨攟梴塼拞偱峴偆丅

Steinberg solution乮100%乯

adjust to pH7.4-7.6 with HCl

PS Stock solution (亊200)
dissolve penicilin 20,000unit and streptomycin 1g in 100ml DW
final conc.:100unit penicilin, 5mg streptomycin /ml
偦偺懠偺攟梴塼

Marc's Modified Ringers (MMR)

conc.(mM)

(g/l)

X10 Stock
(g/l)

MW

NaCl

100

5.844

58.44

58.44

KCl

2

0.149

1.49

74.55

MgCl2�6H2O
(MgSO47H2O)

1

0.203
0.246

2.03
2.46

203.30
(246.48)

CaCl2�2H2O

2

0.294

2.94

147.01

HEPES

5

1.192

11.92

238.3

Adjust pH to 7.5 with 10N NaOH (ca1.2ml)

丂

Modified Barth's Salin(MBS)

Regents

MW

NaCl

88mM

5.14g/l

58.44

KCl

1mM

0.075g

74.55

CaCl2

0.7mM

0.078g

110.99

MgSO4

1mM

0.245g

246.48(7H2O)

HEPES

5mM

1.19g

238.31

NaHCO3

2.5mM

0.21g

84.02

Normal Amphibian medium (NAM)

Regents

NaCl

110mM

KCl

2mM

Ca(NO)3

1mM

MgSO4

1mM

Na2EDTA

0.1mM

NaHCO3

1mM

Na Phosphate

2mM

幚尡宍懺妛